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Rub5 sanasto Flashcards Quizlet

Although the mechanisms by which the expression of ABI5 is regulated by ABI3 are unknown, a key observation of our work is that ABI5 can complement abi3‐1, whereas ABI3 cannot complement abi5‐4. This strongly suggests that ABI5 acts downstream of ABI3 to arrest growth of germinated embryos by ABA. Disruption of ABI5 increases Arabidopsis ABA‐insensitivity and decreases the expression of many ABA‐responsive genes (Finkelstein and Lynch, 2000b), whereas ABI5‐overexpressing plants were hypersensitive to ABA during seed germination and early seedling development (Lopez‐Molina et al., 2001). WTy35S::HA-ABI5 transgenic seedlings (T3 generation) were treated in liquid MS medium with [32P]orthophosphoric acid [100 mCi (1 Ci 5 37 GBq), 285.5 Ciymg, NEN] for 90 min. Seedlings were further treated with or without 50 mM ABA for 30 min.

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ABI5 is a key component in ABA-triggered pathways during germination, seedling establishment, and vegetative growth (Lopez-Molina et al., 2001); in addition, it has been reported to play certain roles in nitrogen assimilation and signaling (Signora et al., 2001). Characterization of abi5 mutants in a second legume species, pea (Pisum sativum), confirmed a role for ABI5 in the regulation of longevity, seed degreening, and RFO accumulation, identifying ABI5 as a prominent regulator of late seed maturation in legumes. The ABI5 subgroup consists 47of ABA INSENSITIVE 5 (ABI5, also referred to as Dc3 PROTEIN BINDING FACTOR or DPBF1) and three other DPBFs48 (DBPF2, DPBF3/AREB3 for ABA RESPONSIVE ELEMENT BINDING, available under aCC-BY-NC-ND 4.0 International license.

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(A) Genotyping and (B) expression analysis of PPC1, PPC2, and 2 Feb 2021 Drought stress analysis of MAP kinase mutants and transgenic plants suggested that except phosphor-null ABI5, all other mutants showed such as ABA-Insensitive5 (ABI5) and ABA-responsive element. Binding Factor3 This review focuses on the role of ubiquitination in.

In this study, we generated a genome‐wide DNA‐binding map of ANAC060, indicating that it directly binds to a G‐box motif. ANAC060 binds to the ABI5 promoter and represses ABI5 transcription, rendering seedling growth insensitive to sugar and ABA. 2014-02-27 · ABI5 expression is greatly reduced in the abi3 mutants, and 35S:ABI5 could rescue ABA insensitivity of abi3. Whether ABI3 is also a direct regulator of ABI5 awaits further investigation. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. 2015-10-23 · GUS staining of ABI5 pro:ABI5-GUS seeds and seedlings was performed as previously described 31 using 50 mM potassium phosphate buffer (pH 7.0) containing 0.05% (v/v) Triton X-100, 1 mM K 3 Fe(CN We used abi5-4 lines expressing the 35S∷ABI5 transgene (abi5-4/35S∷ABI5) to investigate the relationship between protein accumulation and the extent of growth arrest in response to ABA. Fig. 5D shows that abi5-4/35S∷ABI5 transgenic lines expressing intermediate ABI5 levels also displayed an intermediate phenotype in the presence of ABA. The preservation of our genetic resources and production of high-quality seeds depends on their ability to remain viable and vigorous during storage.
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Abstract ABA Insensitive 5 (ABI5) is a basic leucine zipper transcription factor that plays a key role in the regulation of seed germination and early seedling growth in the presence of ABA and abiotic stresses. ABI5 is a key component in ABA-triggered pathways during germination, seedling establishment, and vegetative growth (Lopez-Molina et al., 2001); in addition, it has been reported to play certain roles in nitrogen assimilation and signaling (Signora et al., 2001). Characterization of abi5 mutants in a second legume species, pea (Pisum sativum), confirmed a role for ABI5 in the regulation of longevity, seed degreening, and RFO accumulation, identifying ABI5 as a prominent regulator of late seed maturation in legumes. The ABI5 subgroup consists 47of ABA INSENSITIVE 5 (ABI5, also referred to as Dc3 PROTEIN BINDING FACTOR or DPBF1) and three other DPBFs48 (DBPF2, DPBF3/AREB3 for ABA RESPONSIVE ELEMENT BINDING, available under aCC-BY-NC-ND 4.0 International license.

To identify potential nuclear localization and export signal, the complete amino acid sequence of ABI5 was examined by 12 May 2020 RNA-seq analysis also uncovered differentially regulated genes The study showed that ABI5, which acts at the core of ABA signaling, 30 Nov 2019 In the present review, we focus on two aspects of ABA signaling. seed germination and seedling growth [72,73], which indicates ABI5 acts as Add review · Customer's grade at ABI5 | Abscisic acid insensitive 5 (peptide antibody) in the group. How to cite this product: Product name, number (Agrisera, The gibberellic acid signaling repressor RGL2 inhibits Arabidopsis seed germination by stimulating abscisic acid synthesis and ABI5 activity.
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9F, G). Characterization of abi5 mutants in a second legume species, pea (Pisum sativum), confirmed a role for ABI5 in the regulation of longevity, seed degreening, and RFO accumulation, identifying ABI5 as a prominent regulator of late seed maturation in legumes. ABA-induced flavonol biosynthesis involves HY5, ABI5, and PFG genes.

Rub5 sanasto Flashcards Quizlet

Whether ABI3 is also a direct regulator of ABI5 awaits further investigation. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. 2015-10-23 · GUS staining of ABI5 pro:ABI5-GUS seeds and seedlings was performed as previously described 31 using 50 mM potassium phosphate buffer (pH 7.0) containing 0.05% (v/v) Triton X-100, 1 mM K 3 Fe(CN We used abi5-4 lines expressing the 35S∷ABI5 transgene (abi5-4/35S∷ABI5) to investigate the relationship between protein accumulation and the extent of growth arrest in response to ABA. Fig. 5D shows that abi5-4/35S∷ABI5 transgenic lines expressing intermediate ABI5 levels also displayed an intermediate phenotype in the presence of ABA. The preservation of our genetic resources and production of high-quality seeds depends on their ability to remain viable and vigorous during storage.

Our results show that ABI5 plays a key role in seedling growth under photorespiratory conditions by regulating photorespiratory enzyme expression, and ABA regulates plant growth under low CO 2 conditions through modulating ABI5 expression. abi5-1 seedlings grown at low CO 2 showed lower F v /F m values (Fig. 6C, D). Genetic and physiological evidence has demonstrated this key role of ABI5 in this process. Overexpression of ABI5 resulted in enhanced sensitivity to ABA treatment, and the abi5 mutant was insensitive to ABA treatment during both seed germination and vegetative growth (Finkelstein & Lynch, 2000; Lopez‐Molina et al., 2001; Tezuka et al., 2013).